Phylogenomic Analysis Reveals Origin, Spread of MRSA Clone
(HealthDay News) – Phylogenomic analysis has revealed details about the emergence and transmission of a major methicillin-resistant Staphylococcus aureus (MRSA) clone, EMRSA-16.
Paul R. McAdam, from the University of Edinburgh in the United Kingdom, and colleagues performed a Bayesian phylogenetic reconstruction on the basis of genome sequences from 87 Staphylococcus aureus isolates. The isolates were collected from patients in three continents over a 53-year period and included 60 isolates of the pandemic EMRSA-16 clone and 27 additional clonal complex 30 (CC30) isolates.
The researchers found that there was a common ancestor shared by the three major pandemic clones, originating from the CC30 lineage (phage type 80/81, Southwest Pacific, and EMRSA-16), which existed more than 100 years ago. In contrast, the hospital-associated EMRSA-16 clone likely emerged 35 years ago. A genome-wide analysis of CC30 revealed molecular correlates of hospital- or community-associated pandemics, including mobile genetic elements and nonsynonymous mutations impacting both antibiotic resistance and virulence. Phylogeographic analysis demonstrated that the spread of EMRSA-16 within the United Kingdom was from hospitals in large population centers in London and Glasgow to regional health care settings.
"Taken together, the high-resolution phylogenomic approach used resulted in a unique understanding of the emergence and transmission of a major MRSA clone and provided molecular correlates of its hospital adaptation," the authors write.
ARK-Genomics at the Roslin Institute performed sequencing services for the study.