Blood and Saliva to Predict Return of HPV-Related Oral Cancer
Physicians have developed blood and saliva tests that help accurately predict recurrences of HPV-linked oral cancers in a substantial number of patients. The tests screen for DNA fragments of the human papillomavirus (HPV) shed from cancer cells lingering in the mouth or other parts of the body.
"There is a window of opportunity in the year after initial therapy to take an aggressive approach to spotting recurrences and intensively addressing them while they are still highly treatable," said senior author Joseph Califano, MD, professor of Otolaryngology - Head and Neck Surgery, member of the Johns Hopkins Kimmel Cancer Center, and medical director of the Milton J. Dance Jr. Head and Neck Center at the Greater Baltimore Medical Center in Maryland. He added, "Until now, there has been no reliable biological way to identify which patients are at higher risk for recurrence, so these tests should greatly help do so.”
Patients with HPV-associated oropharyngeal cancers are generally examined every 1 to 3 months in the first year after diagnosis. Recurrences are often found when patients report ulcers, pain, or lumps in the neck. But imaging tests are unreliable in detecting cancer recurrence earlier, and the location of oropharyngeal cancers, which is in the tonsils, throat, and base of the tongue, make it difficult for physicians to spot budding lesions.
Califano said survival rates for patients with early stage, HPV-related oral cancers are as high as 90% within the first 2 years, and a study reported by Johns Hopkins experts in February showed that, even after recurrence, more than 50% of patients survive 2 years after their recurrence. He explained that the new blood and saliva tests have the potential to improve these rates.
For the study, published in JAMA Otolaryngology – Head & Neck Surgery (2014; doi:10.1001/jamaoto.2014.1338), the researchers analyzed blood and saliva samples from 93 patients with oropharyngeal cancer who were treated with surgery, radiation alone, or combined chemotherapy and radiation at The Johns Hopkins Hospital or Greater Baltimore Medical Center. Samples were collected before and after treatment. Some 81 patients had HPV-positive tumors. The researchers selected patients with a variety of early-to-advanced stage cancers; none of the patients had distant metastasis.
The scientists found that HPV DNA detected in patients' saliva after treatment was predictive for recurrence nearly 20% of the time in a subset of the patients. When the scientists looked for HPV DNA in the blood of another subset of patients, the accuracy of a recurrence prediction rose to more than 55%. In a third subset of patients, finding HPV DNA in both blood and saliva samples after treatment accurately predicted recurrence 70% of the time.
Despite the encouraging results, Califano said, further refinements are still badly needed to improve detection of possible recurrences because HPV is highly prevalent in our bodies, and "we can't be sure our test results are cancer-specific and not due to other forms of HPV infection or exposure."